|Flow Cytometry (FACS)||1:50-1:100|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Tested Species reactivity||Pig|
|Host / Isotype||Mouse / IgG1|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
Studies suggest that CD203a migrates as a homodimer of ~260kDa under non-reducing conditions and a 130kDa monomer under reducing conditions from preparations of porcine alveolar macrophages. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Porcine CD203a, originally clustered as SWC9, was later identified as the porcine homologue of human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 or NPP1. CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages, and is not expressed on monocyte populations. SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), is expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation.
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