|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:100|
|Immunohistochemistry (IHC)||See 3 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding human CD23 48-248aa|
|Storage buffer||tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
CD23 is a 45kDa glycoprotein, which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BLAST-2; C-type lectin domain family 4; C-type lectin domain family 4 member J; C-type lectin domain family 4, member J; CD23; CD23 antigen; CD23A; CLEC4J; Fc epsilon receptor II; Fc fragment of IgE, low affinity II, receptor for (CD23); Fc-epsilon-RII; FCE2; IGEBF; Immunoglobulin E-binding factor; immunoglobulin epsilon-chain; Low affinity immunoglobulin epsilon Fc receptor; Lymphocyte IgE receptor; member J
Gene Aliases: BLAST-2; CD23; CD23A; CLEC4J; FCE2; FCER2; IGEBF
UniProt ID: (Human) P06734
Entrez Gene ID: (Human) 2208
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