|Flow Cytometry (Flow)||5 µl for 10^6 cells in 100 µl|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Flow Cytometry (Flow)||See 2 publications below|
|Tested Species reactivity||Human, Non-human primate|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human CD28 transfected into murine T cell hybridoma.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage conditions||4° C|
MA1-20792 detects CD28 in human and non-human primate samples.
MA1-20792 has been successfully used in IF, FACS, immunocytochemistry, and immunohistochemistry (frozen) procedures.
The MA1-20792 immunogen is human CD28 transfected into mouse T cell hybridoma.
Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD28; CD28 antigen; MGC138290; T-cell-specific surface glycoprotein CD28; TP44
Gene Aliases: CD28; Tp44
UniProt ID: (Human) P10747
Entrez Gene ID: (Human) 940
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