|Flow Cytometry (Flow)||Assay Dependent|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||human thymocytes and PHA-activated human T-lymphocytes|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 0.2% carrier protein|
|Contains||0.08% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is a monoclonal mixture of FITC-conjugated CD3 (clone M2AB, isotype IgG1) and PE-conjugated CD25 (clone ITYV, isotype IgG1).
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. Natural killer (NK) cells in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. NK cells are important effectors of innate immunity where they release cytokines, which in turn up-regulate other immunological functions. Monoclonal antibodies have been used to identify different surface antigens present on NK cells. These surface antigens have not only been used to identify NK cells, but also their functionally distinct subsets. The Cluster of Differentiation (CD) nomenclature was established to standardize the naming of NK cells. To date, a total of 151 CD clusters and subclusters have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD25; CD3 antigen, delta subunit; CD3 delta; CD3 epsilon; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); epsilon polypeptide TiT3 complex; epsilon subunit of T3; FLJ18683; GAS-3; Growth arrest-specific protein 3; IL-2 receptor subunit alpha; IL-2R alpha; IL-2R alpha chain; IL-2R subunit alpha; IL2 RA antibody; Il2ra antibody; Interleukin 2 receptor alpha chain antibody; interleukin 2 receptor, alpha; Interleukin-2 receptor subunit alpha; OKT3, delta chain; p55; Peripheral myelin protein 22; peripheral myelin protein 22 kDa; PMP-22; T-cell antigen receptor complex; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T3E; TAC antigen; TAC antigen antibody; TCRE
Gene Aliases: CD25; CD3-DELTA; CD3-GAMMA; CD3D; CD3E; CD3G; CMT1A; CMT1E; DSS; GAS-3; GAS3; HMSNIA; HNPP; IDDM10; IL2R; IL2RA; IMD17; IMD18; IMD19; IMD41; p55; PMP22; Sp110; T3D; T3E; T3G; TCGFR; TCRE
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