|Flow Cytometry (Flow)||Assay Dependent|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Clone||M2AB, 7E14, BHPT-1|
|Immunogen||human thymocytes, human peripheral blood T lymphocytes and peripheral blood monocytes from a rheumatoid arthritis patient|
|Conjugate||PE , PE-Cyanine5|
|Storage buffer||PBS with 0.2% carrier protein|
|Contains||0.08% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is a monoclonal mixture of FITC-conjugated CD3 (clone M2AB, isotype IgG1), PE-conjugated CD4 (clone 7E14, isotype IgG1) and Cy5-PE-conjugated CD45 (clone BHPT-1, isotype IgG1).
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.. The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils.. CD45 Leukocyte Common Antigen (LCA) belongs to the family of at least four isoforms of membrane glycoproteins (220, 205, 190, 180 kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. The intracellular portion of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B220; c polypeptide; CD3 antigen, delta subunit; CD3 delta; CD3 epsilon; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; CD45; CD45 antigen; CD45R; epsilon polypeptide TiT3 complex; epsilon subunit of T3; FLJ18683; GP180; L-CA; LCA; Leukocyte common antigen; LY5; OKT3, delta chain; protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor type; Receptor-type tyrosine-protein phosphatase C; T-cell antigen receptor complex; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD4; T200; T200 glycoprotein; T200 leukocyte common antigen; T3E; TCRE
Gene Aliases: B220; CD3-DELTA; CD3-GAMMA; CD3D; CD3E; CD3G; CD4; CD45; CD45R; CD4mut; GP180; IMD17; IMD18; IMD19; L-CA; LCA; LY5; PTPRC; T200; T3D; T3E; T3G; TCRE
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