|Flow Cytometry (FACS)||1:20|
|Immunocytochemistry (ICC)||1:20 - 1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:20|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MCF7 MR cell line, over-expressing ABCG2.|
|Storage buffer||tissue culture supernatant with 1% BSA|
|Contains||0.1% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-35030 detects ABCG2 in human samples. BXP-34 did not cross react with the human MDR1, MRP1, MRP2, MRP5 gene products.
Permeabilization is necessary for flow cytometry applications. This antibody is not recommended for Western blot. For IHC applications, only acetone-fixed frozen sections should be used; this antibody does not react with paraffin-embedded tissues. ICC was performed using acetone-fixed cytospin preparations.
The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ABC transporter; ATP-binding cassette sub-family G member 2; ATP-binding cassette transporter G2; breast cancer resistance protein; mitoxantrone resistance-associated protein; multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2; placenta specific MDR protein; placenta-specific ATP-binding cassette transporter
Gene Aliases: ABC15; ABCP; BCRP; BCRP1; BMDP; CD338; CDw338; EST157481; GOUT1; MRX; MXR; MXR1; UAQTL1
UniProt ID: (Human) Q4W5I3
Entrez Gene ID: (Human) 9429
Molecular Function: Transporter
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