|Flow Cytometry (Flow)||1:50|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||Assay-dependent|
|Immunohistochemistry (IHC)||See 21 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human, Non-human primate|
|Published species reactivity||Rabbit , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human endothelial vesicles|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody detects CD34 in human, Cynomolgus monkey and Rhesus monkey samples.
IHC was performed using methanol-fixed cells. Do not perform antigen retrieval prior to IHC staining. This target has a predicted molecular weight of 40 kDa.
CD34 is possibly a adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD34; CD34 antigen; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2
Gene Aliases: CD34
UniProt ID: (Human) P28906
Entrez Gene ID: (Human) 947
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