|Flow Cytometry (Flow)||Neat|
|Tested Species reactivity||Dog|
|Host / Isotype||Mixed / IgG1|
|Immunogen||Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-9, Canine CD8 alpha chimaeric human IgG1 Fc fusion protein.|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 1% BSA, 5% sucrose|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
This antibody recognizes canine CD3 epsilon and CD8 cell surface antigens. Clone CA17.2A12 is a FITC-conjugated mouse IgG1 that recognizes canine CD3, a T cell differentiation antigen expressed on mature T lymphocytes. Clone YCATE 55.9 is an RPE-conjugated Rat IgG1 that recognizes canine CD8 epsilon, which is expressed by cytotoxic T cells. YCATE 55.9 binds to CD8 alpha.
Reconstitute with 1 ml of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD3 epsilon; CD3-epsilon; CD3e; CD3e antigen; CD3E antigen, epsilon polypeptide (TiT3 complex); CD8; CD8 antigen, alpha polypeptide (p32); CD8a; CD8alpha; CD8beta; epsilon polypeptide TiT3 complex; epsilon subunit of T3; FLJ18683; Leu-2; MAL; T-cell antigen receptor complex; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD8 alpha chain; T3E; TCRE
Gene Aliases: CD3E; CD8A
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