|ELISA (ELISA)||1 µg/ml|
|Flow Cytometry (Flow)||1:50-1:100|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||Assay dependent|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide corresponding to the 113 amino acid residue cytoplasmic domain of murine zeta.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.09% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-20241 detects TCR zeta chain in human and mouse samples.
MA1-20241 has been successfully used in ELISA, FACS, immunohistochemistry (frozen), immunoprecipitation, and Western blot procedures.
The MA1-20241 immunogen is a synthetic peptide corresponding to the 113 amino acid residue cytoplasmic domain of mouse zeta.
The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits, all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD247; CD247 antigen; CD247 antigen, zeta subunit; CD3 antigen, zeta polypeptide; CD3-ZETA; CD3-zeta/eta; CD3H; CD3Q; CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 eta chain; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; T3Z; TCR zeta chain; TCRZ
Gene Aliases: 4930549J05Rik; A430104F18Rik; AW552088; CD247; Cd3; Cd3-eta; CD3-ZETA; CD3H; CD3Q; CD3Z; Cd3zeta; IMD25; T3Z; Tcrk; TCRZ
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.