|Flow Cytometry (Flow)||1:25-1:100|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Tested Species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rat thymocyte membrane glycoproteins|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
Heat-mediated antigen retrieval is recommended for the staining of paraffin sections. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: galactoglycoprotein; GPL115; leukocyte sialoglycoprotein; leukosialin; leukosianin; LSN; sialophorin gpL115; W3/13 antigen
Gene Aliases: Cd43; gpL115; Lsn; Lsn1
Entrez Gene ID: (Rat) 24796
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.