|Flow Cytometry (Flow)||1/25 -1/100|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1/100 - 1/200|
|Immunoprecipitation (IP)||Assay Dependent|
|Blocking Assay (BLOCK)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
This antibody recognizes an epitope that is common to all CD44 isoforms.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD44; CD44 antigen; CDw44; Cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing adhesion receptor; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronate binding protein; Hyaluronate receptor; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; soluble CD44
Gene Aliases: CD44; CDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1
UniProt ID: (Human) P16070
Entrez Gene ID: (Human) 960
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