|Flow Cytometry (Flow)||1:50-1:100|
|Functional Assay (FN)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:5-1:10|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Human|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Human Peripheral Blood T cells.|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is placenta. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.
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Protein Aliases: 16.3A5; 1F5; 1F5 antigen; 20 kDa homologous restriction factor; CD59; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); CD59 glycoprotein; CD59 molecule complement regulatory protein; CD59 molecule, complement regulatory protein; EJ16; EJ30; EL32; G344; HRF-20; HRF20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MAC-inhibitory protein; MAC-IP; MACIF; MEM43; MEM43 antigen; membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane inhibitor of reactive lysis; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20; Protectin; surface anitgen recognized by monoclonal antibody 16.3A5; T cell-activating protein
Gene Aliases: 16.3A5; 1F5; CD59; EJ16; EJ30; EL32; G344; HRF-20; HRF20; MAC-IP; MACIF; MEM43; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20
UniProt ID: (Human) P13987
Entrez Gene ID: (Human) 966
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