|Flow Cytometry (Flow)||Neat-1:10|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||THP1 (human acute monocytic leukaemia cells)|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS with 1% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred, store in dark|
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
This type 2 fucosylated carbohydrate antigen is expressed on the surface of all myeloid cells during development. Moreover, CD65 is expressed highly on granulocytes and weakly on monocytes in peripheral blood. CD65 has been reported to be a ligand for CD62E (E-Selectin). However, the sialylated form of CD65 (designated CD65s) can be used as a marker for acute myeloid leukemia (AML) as its expression increases as CD34 decreases. Finally, CD65 is important for extravascular infiltration of AML cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Fucoganglioside Type 2; Fucoganglioside Type II; sialylated CD65
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