|Flow Cytometry (Flow)||Assay Dependent|
|Flow Cytometry (Flow)||See 1 publications below|
|Tested Species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Purified Concanavalin A acceptor glycoprotein from P815 cell line|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred, store in dark|
Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD, which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation. MA1-82739 does detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD68; CD68 antigen; LAMP4; macrophage antigen CD68; Macrosialin; SCARD1; scavenger receptor class D member 1
Gene Aliases: Cd68; gp110; Lamp4; Scard1
UniProt ID: (Mouse) P31996
Entrez Gene ID: (Mouse) 12514
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