|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 211 and 538 of Human CD73|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-27336 targets CD73 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-27336 immunogen is recombinant fragment corresponding to a region within amino acids 211 and 538 of Human CD73.
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 220.127.116.11) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 5'-NT; 5'-nucleotidase; 5'-nucleotidase, ecto (CD73); CD73; E5NT; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NTE; Purine 5-Prime-Nucleotidase
Gene Aliases: CALJA; CD73; E5NT; eN; eNT; NT; NT5; NT5E; NTE
UniProt ID: (Human) P21589
Entrez Gene ID: (Human) 4907
Molecular Function: phosphodiesterase
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