|Flow Cytometry (Flow)||1 ug/1 million cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Clone||YTS 169AG 101HL|
|Immunogen||Mouse Ly-2 thymocytes.|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-70041 detects CD8a in mouse samples.
MA1-70041 has been successfully used in flow cytometry and immunohistochemistry (PFA-fixed paraffin and frozen) procedures. PFA-fixed paraffin-embedded tissue sections require antigen retrieval prior to immunostaining.
The MA1-70041 immunogen is mouse Ly-2 thymocytes.
Store at 4°C for short term. For extended storage aliquot and store at -20°C. Avoid Freeze/Thaw Cycles.
MA1-70041 reacts with a protein of approximately 30 kDa found on mouse thymocytes and mouse cytotoxic/suppressor T cells. It does not bind to mouse helper/inducer T cells. It binds to T lymphocytes from all mouse strains regardless of phenotypic expressin (i.e. reacts with T lymphocytes from mouse strains expresssing the Ly2.1 or Ly2.2 phenotype). It can be used to investigate the role of T cells in models for infectious disease, autoimmunity transplantation tolerance and fundamental aspects of immunology. It can also be useful to identify/eliminate cytotoxic or suppressor T lyphocytes in vivo or in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD8; CD8a; CD8alpha; CD8b; CD8beta; Leu-2; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); MAL; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3
Gene Aliases: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.