|Flow Cytometry (Flow)||1:100 -1:200|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Tested Species reactivity||Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rat / IgG2a|
|Immunogen||T cell clone, C6|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD8 is expressed on T-cells and exists either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. CD8 functions as a co-receptor with the T-cell receptor and mediates efficient cel-to-cell interactions within the immune system. The CD8 alpha chain specifically binds to class I MHC molecules. CD8 alpha is expressed by a subset of T lymphocytes, most thymocytes, and the majority of NK cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD8; CD8a; Leu-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); MAL; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2
Gene Aliases: BB154331; Cd8a; Ly-2; Ly-35; Ly-B; Lyt-2; Lyt2
UniProt ID: (Mouse) P01731
Entrez Gene ID: (Mouse) 12525
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