|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1/10 - 1/50|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Mouse, Rat|
|Host / Isotype||Armenian hamster / IgG1|
|Immunogen||38C13, murine B cell line.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
This antibody induces homotypic aggregation of B cells and inhibits anti-Ig and IL-4 induced proliferation, and requires the presence of both extracellular loops of TAPA-1 for binding.
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD81 is a 26kD cell surface glycoprotein that is also known as TAPA-1. In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 26 kDa cell surface protein TAPA-1; CD 81 antigen; CD81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); target of antiproliferative antibody 1; Target of the antiproliferative antibody 1; tetraspanin-28; tspan-28
Gene Aliases: Cd81; Tapa-1; Tapa1; Tspan28
UniProt ID: (Mouse) P35762
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.