|ELISA (ELISA)||0.1-1.0 ug/ml|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||5-10 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Immunoprecipitation (IP)||5 ug|
|Western Blot (WB)||0.5-2 µg/ml|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Tested Species reactivity||Amphibian, Chicken, Human, Mouse, Rat|
|Published species reactivity||Mouse Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Recombinant protein consisiting of the carboxy-terminal two-thirds of the Xenopus p34cdc2|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor , which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CDC2; CDC28A; CDK1; CDKN1; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; Cell division control protein 2 homolog; cell division cycle 2 homolog A; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; Cell division protein kinase 1; Cyclin-dependent kinase 1; p34 protein kinase; P34CDC2
Gene Aliases: CDC2; CDC28A; Cdc2a; CDK1; CDKN1; p34<CDC2>; P34CDC2
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