|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
|Western Blot (WB)||See 4 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified recombinant CDC25A|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage conditions||4° C|
MA5-13794 targets CDC25A in IP, IHC (P) and WB applications and shows reactivity with Human and Rat samples.
The MA5-13794 immunogen is purified recombinant CDC25A.
Cyclin-dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin-dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S-phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CDC25 isoform A1-CAG; CDC25A2-CAG isoform; cell division cycle 25A; Dual specificity phosphatase Cdc25A; M-phase inducer phosphatase 1
Gene Aliases: CDC25A; CDC25A2; Cdc25m3; D9Ertd393e
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