|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminus of human PITSLRE|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 110 and 92 kDa.
Purity is >95% by SDS-PAGE.
The PITSLRE beta1 protein, a distantly related member of the Cdk family of protein kinases, induces apoptosis after low levels of ectopic expression. Apoptosis, or programmed cell death, is similarly induced by ectopic expression of an amino terminal deletion mutant retaining the catalytic and carboxyterminal domains of PITSLRE beta1, but not by other mutants lacking Histone H1 kinase activity or by other Cdk family members. The terminology for the ten isoforms of the PITSLRE subfamily of proteins is based on the conserved PSTAIRE box region of Cdc2 p34. Depending on which of the PITSLRE genes produce the protein, the cDNA and protein are designated alpha, beta or gamma (i.e., PITSLRE A gene, alpha; PITSLRE B gene, beta and PITSLRE C gene, gamma). Some of the isoforms such as PITSLRE alpha1 (T cells) and PITSLRE beta1 (B cells and brain), are expressed in specific cell types, while others are expressed ubiquitously.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CDC-related protein kinase p58; CDC2L2; CDC2L3; CDK11; CDK11 p110; CDK11 p46; CDK11 p58; CDK11A; CDK8 like cyclin dependent kinase; cell division cycle 2 homolog-like 1; cell division cycle 2 homolog-like 2; Cell division cycle 2 like 2 (PITSLRE proteins); Cell division cycle 2 like protein kinase 2; cell division cycle 2-like 1; cell division cycle 2-like 1 (PITSLRE proteins); cell division cycle 2-like 2; Cell division cycle 2-like protein kinase 1; Cell division protein kinase 11; Cell division protein kinase 11B; CLK-1; Cyclin dependent kinase (CDC2 like) 11; Cyclin-dependent kinase 11; Cyclin-dependent kinase 11B; Death preventing kinase; Galactosyltransferase associated protein kinase p58/GTA; Galactosyltransferase-associated protein kinase p58/GTA; p5; p58 CLK-1; PITSLRE proteins; PITSLRE serine/threonine-protein kinase CDC2L1
Gene Aliases: AA989746; Cdc11b; CDC2L1; Cdc2l2; CDK11; CDK11-p110; CDK11-p46; CDK11-p58; CDK11B; CLK-1; p58; p58CDC2L1; p58CLK-1; PITSLREA; PK58
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