|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||Assay-Dependent|
|Immunofluorescence (IF)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Tag Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Purified recombinant human cdk2 protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissues in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at room temperature for 20 min. Recommended positive controls include HeLa cells and tonsil tissue.
p33cdk2 associates with cyclin A in human cells. Kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important (as does cyclin A-cdk2) for the progression from G1-to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cdc2-related protein kinase; CDC28; CDC2A; Cell division protein kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; EC 188.8.131.52; EC 184.108.40.206; kinase Cdc2; MPF; p33 protein kinase
Gene Aliases: A630093N05Rik; CDK2; CDKN2; p33(CDK2)
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