|Flow Cytometry (Flow)||Assay-Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay-Dependent|
|Western Blot (WB)||See 5 publications below|
|Miscellaneous PubMed (MISC)||See 9 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Immunohistochemistry (IHC)||See 5 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 3 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Tag , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant cdk4 protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
This antibody reacts with an epitope at aa 16-35.
Recommended positive controls include MAD109, LS174T, and PC12 cells.
CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of the gene have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cell division protein kinase 4; CRK3; cyclin dependent kinase 4; Cyclin-dependent kinase 4; p34<PSK-J3>/cdk4; PSK-J3; serine/threonine kinase
Gene Aliases: CDK4; CMM3; Crk3; PSK-J3
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