|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to N-terminus of human CDX2.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is colon adenocarcinoma.
CDX2 is a caudal type homeobox gene that encodes an intenstine-specific transcription factor that is expressed early in intestinal development and may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells. It is expressed in the nuclei of epithelial cells throughout the intestine, from duodenum to rectum. The CDX2 protein is expressed in primary and metastatic colorectal carcinomas and has also been demonstrated in the intestinal metaplasia of the stomach and intestinal-type gastric cancer, while it is not expressed in the normal gastric mucosa. Studies have shown that CDX2 is superior marker compared in CK20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: caudal type homeo box transcription factor 2; caudal type homeobox transcription factor 2; Caudal-type homeobox protein 2; CDX-3; CDX3; Homeobox protein CDX-2; homeobox protein miniCDX2
Gene Aliases: CDX-3; CDX2; CDX2/AS; CDX3
UniProt ID: (Human) Q9UD92
Entrez Gene ID: (Human) 1045
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