|Flow Cytometry (Flow)||1 µg/10^6 cells|
|Immunoprecipitation (IP)||3 ug/ml|
|Western Blot (WB)||0.5-1 ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human CENP-E|
|Contains||0.1% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This target has a predicted molecular weight of 312 kDa and is only suitable for Western blot if IP is performed first.
The kinetophore is a macromolecular structure that is associated with the centromeres of chromosomes and is responsible for establishing and maintaining the connections with the microtubules of the mitotic spindle. The proteins that reside within the chromosomal domain fall into two broad classes: those that associate with the centromere throughout the cell cycle and those that are found at the centromere in a cell cycle specific manner. Members of the first class include CENP-A, CENP-B, and CENP-C. The members of the second class include several ubiquitous cellular proteins such as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F. CENP-F appears to be the earliest member to interact with the centromere-kinetochore complex. CENP-F also shows a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural components it localizes to and therefore may play a role in several mitotic events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CENP-E; Centromere autoantigen E; Centromere autoantigen E (312kD); Centromere protein E; Centromere protein E 312kDa; centromere protein E, 312kDa; Centromere-associated protein E; Kinesin family member 10; Kinesin related protein CENPE; Kinesin-7; Kinesin-related protein CENPE; protein phosphatase 1, regulatory subunit 61
Gene Aliases: CENP-E; CENPE; KIF10; MCPH13; PPP1R61
UniProt ID: (Human) A6NKY9
Entrez Gene ID: (Human) 1062
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