|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||0.05-0.1 µg/ml|
|Tested Species reactivity||Bovine, Chicken, Human, Mouse, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (68 KPLSQEELQRIKNEKKQK 85) of CNOT4 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
CNOT4 is a 575 amino acid protein with E3 ubiquitin ligase activity, mainly involved in protein modification and protein ubiquitination. It contains a ring type zinc finger domain of the C4C4 type, an RNA recognition motif and a bipartite nuclear localization signal. Studies show that CNOT4 is a component of the CCR4-NOT (CAF1) core complex that functions as general transcription regulation complex and has both a positive as well as a negative effect on the transcription of multiple functionally unrelated genes, thus controls gene expression. CNOT4 interacts with CNOT1 via C-terminus and with E2 ubiquitin ligases via C4C4 RING domain. Reports suggest that CNOT4 gets phosphorylated in response to DNA damage on consensus sites recognized by ATM and ATR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CCR4-associated factor 4; CCR4-NOT transcription complex subunit 4; CCR4-NOT transcription complex, subunit 4; CLONE243; E3 ubiquitin-protein ligase CNOT4; negative regulator of transcription 4; NOT4; NOT4 (negative regulator of transcription 4; NOT4 (negative regulator of transcription 4, yeast) homolog; NOT4H; Potential transcriptional repressor NOT4Hp; RING-type E3 ubiquitin transferase CNOT4; transcriptional repressor NOT4Hp; yeast) homolog
Gene Aliases: BOS_4552; CLONE243; CNOT4; NOT4; NOT4H; Not4hp; RCJMB04_19d21
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