|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1-2ug/test|
|Immunohistochemistry (Frozen) (IHC (F))||1:250|
|Immunohistochemistry (Paraffin) (IHC (P))||1/1000|
|Western Blot (WB)||1:1,000|
|Western Blot (WB)||See 5 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Tested Species reactivity||Bovine, Chicken, Cat, Human, Mouse, Non-human primate, Rabbit, Reptile, Rat|
|Published species reactivity||Avian , Rat , Human , Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Oxidized CRABPI from bovine retina.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-813 detects cellular retinoic acid binding protein I (CRABPI) from human, mouse, monkey, feline, bovine, rabbit, rat, turtle and chicken tissues. This antibody does not react with chameleon, goldfish and frog. This antibody does not cross-react with cellular retinol binding protein (CRBP), performic acid oxidized CRBP, interphotoreceptor retinoid binding protein or retinol binding protein.
MA3-813 has been successfully used in Western blot, immunohistochemistry and ELISA procedures. By Western blot, this antibody detects a 16 kDa protein representing CRABPI from rat retinal supernatant. Immunohistochemical staining of CRABPI in rat retina with MA3-813 results in its localization to amacrine somata and laminae in the inner plexiform layer.
The MA3-813 antigen is oxidized CRABPI from bovine retina.
Retinoic acid (RA), a metabolite of vitamin A, plays an important role in growth and differentiation by modulation of expression of certain genes. RA functions through its interaction with the nuclear protein, retinoic acid receptor (RAR) and the 9-cis RA with retinoid X receptor (RXR). The cellular retinoic acid binding proteins (CRABP) bind RA with a high affinity and belong to the family of fatty acid binding proteins.
Two forms of CRABP have been characterized, CRABPI and CRABPII which share ~75% sequence conservation. The main role of CRABPI may be to protect retinoids from non-specific dehydrogenases and direct them to specific enzymes. The precise role of CRABPII is still poorly understood but it seems to play a role in the interaction of ligands (RA, 9-cis RA) with nuclear receptors (RAR/RXR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cellular retinoic acid binding protein 1; cellular retinoic acid binding protein I; cellular retinoic acid binding protein I (CRABP-I) homologue; Cellular retinoic acid-binding protein 1; Cellular retinoic acid-binding protein I; CRABP-I; CRABPI
Gene Aliases: AI326249; BOS_20217; cCRABP-I; CRABP; Crabp-1; CRABP-I; CRABP1; CRABPI; Rbp-5; RBP5
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