|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-3 ug/ml|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminal region of the human casein kinase 1 epsilon protein (Accession# NP_689407), which is identical to mouse and rat sequence.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
Casein kinases are a group of ubiquitous Ser/Thr kinases that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, circadian rhythms, and metabolic pathways. CK1 family members share a highly conserved kinase domain but differ in their variable N-and C-terminal domains. Mammals have seven family members: alpha, beta, gamma1, gamma2, gamma3, delta and epsilon. They all share at least 50% amino acid identity within the protein kinase catalytic domain. CK1 isoforms exclusively use ATP as phosphate donor and are generally co-factor independent. Casein kinase I epsilon is a Wnt-regulated kinase, and regulated phosphorylation of Dvl allows fine tuning of the Wnt-b-catenin signaling pathway. CKIe positively regulates Akt possibly by inhibiting PP2A. CKIe play an important role in neurodegenerative diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: casein kinase 1 epsilon; casein kinase 1, epsilon; casein kinase I epsilon; Casein kinase I isoform epsilon; CK1e; CKI, epsilon; CKI-epsilon; CKIe; HCKIE; KC1 epsilon; MGC10398; OTTHUMP00000063258; OTTHUMP00000063263; RP1-5O6.1
Gene Aliases: AI426939; AI551861; AW457082; CK1epsilon; CKIe; CKIepsilon; Csnk1e; HCKIE; KC1epsilon; tau
UniProt ID: (Mouse) Q9JMK2
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