|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 62-115 of human CUG-BP1/2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 50 and 54 kDa.
Purity is >95% by SDS-PAGE.
CUG-BP1 and CUG-BP2 are proteins that bind specifically to (CUG)8 oligonucleotides in vitro. While CUG-BP1 has the major binding activity in normal cells, nuclear CUG-BP2 binding activity increases in DM cells. Both CUG-BP1 and CUG-BP2 are isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. CUG-BP1, an RNA CUG triplet repeat binding protein, regulates splicing and translation of various RNAs. Expansion of RNA CUG repeats in the DMPK in DM is associated with alterations in binding activity of CUG-BP1 as well as alterations in the translation of the C/EBPb transcription factor. CUG-BP1 is an important regulator of initiation from different AUG codons of C/EBPb mRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 50 kDa nuclear polyadenylated RNA-binding protein; Brain protein F41; bruno-like 2; Bruno-like protein 2; Bruno-like protein 3; BRUNOL2; BRUNOL3; CELF-1; CELF-2; CELF1; CELF2; CUG RNA-binding protein; CUG triplet repeat RNA-binding protein 1; CUG triplet repeat RNA-binding protein 2; CUG triplet repeat, RNA binding protein 1; CUG triplet repeat, RNA binding protein 2; CUG triplet repeat, RNA-binding protein 1; CUG-BP- and ETR-3-like factor 1; CUG-BP- and ETR-3-like factor 2; CUG-BP1; CUG-BP2; CUGBP; CUGBP Elav-like family member 1; CUGBP Elav-like family member 2; Cugbp- and Etr3-like factor 2; CUGBP1; CUGBP2; Deadenylation factor CUG-BP; Deadenylation factor EDEN-BP; EDEN-BP homolog; ELAV-type RNA-binding protein 3; embryo deadenylation element binding protein; Embryo deadenylation element-binding protein homolog; ETR-3; ETR3; hNAPOR; mNapor; NAB50; NAPOR; Neuroblastoma apoptosis-related RNA-binding protein; nuclear polyadenylated RNA-binding protein, 50-kD; RNA-binding protein BRUNOL-2; RNA-binding protein BRUNOL-3; RNA-binding protein Brunol3; rNapor
Gene Aliases: 1600010O03Rik; AA407467; B230218O03; B230345P09Rik; BRUNOL2; BRUNOL3; C88023; CELF-2; CELF1; CELF2; CUG-BP; CUG-BP1; CUG-BP2; CUGBP; CUGBP1; CUGBP2; D230046B21Rik; D2Wsu101e; EDEN-BP; ETR-3; ETR3; HNAB50; mETR-3; NAB50; NAPOR; Napor-2
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.