|ELISA (ELISA)||0.1-1.0 ug/ml|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunoprecipitation (IP)||5-10 ug|
|Western Blot (WB)||1-2 µg/ml|
|Western Blot (WB)||See 10 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Insect , Virus , Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A synthetic peptide corresponding to an 11 amino acid sequence at the C-terminus of the human CUL-1 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
CUL-1 Antibody (2H4C9), Prod# 32-2400 specifically detects neddylated and unmodified Cullin 1 in human cells.
Cullin 1 is a member of the family of human cullin genes (CUL 1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc 53 gene. CUL 1 forms a complex with human p19 skp1 and F box protein p45 skp2, both of which, together with cdc 53 (known as CUL A), as three subunits form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. The CUL 1/p19 skp1/p45 skp2complex is thought to play a role in the ubiquitination of G1 regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CUL-1; Cullin-1
Gene Aliases: CUL1
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