The antibody target was verified by IP Mass Spectrometry to ensure the antibody binds to the antigen stated. View details.
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||2-5 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||human CXCL5 protein corresponding to amino acids 37–114|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
CXCL5 is a member of the CXC subfamily of chemokines. The cDNA encodes a 114 amino acid precursor protein which when cleaved generates a 36 amino acid signal peptide and a 78 amino acid mature protein which contains a 12 amino acids sequence found before the CXC motif. CXCL5 is produced by various human epithelial cells and it binds to the same receptors as IL8 and NAP2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C-X-C motif chemokine 5; chemokine (C-X-C motif) ligand 5; ENA-78; ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; LIX; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; Scyb5; Scyb6; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); Small-inducible cytokine B5
Gene Aliases: CXCL5; ENA-78; ENA78; SCYB5
UniProt ID: (Human) P42830
Entrez Gene ID: (Human) 6374
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.