|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:100|
|Western Blot (WB)||1-2 µg/mL|
|Tested Species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: A(1) V E G P R W K Q A E D I R D I Y D(18)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-543 detects calcium/calmodulin-dependent kinase I (CaMKI) from rat and mouse brain extract.
PA1-543 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~43 kDa protein representing CaMKI from rat and mouse brain extract.
PA1-543 immunizing peptide corresponds to amino acid residues 1-18 from human CaMKI protein. PA1-543 immunizing peptide (Cat. # PEP-134) is available for use in neutralization and control experiments.
Calcium/calmodulin-dependent kinases (CaMKs) are a subfamily of the serine/threonine protein kinase family that includes CaMKI, CaMKII, and CaMKIV. There have been identified multiple substrates for CaMKI, including synapsin 1, synapsin 2, CREB, and CFTR, and its activation is dependent upon phosphorylation of Thr177 by CaMK kinase (CaMKK). Studies suggest that CaMKI plays an important role in retinal function and its expression is regulated by light stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Calcium / calmodulin dependent protein kinase 1; calcium/calmodulin-dependent protein kinase I; Calcium/calmodulin-dependent protein kinase type 1; CaM kinase I; CaM kinase I alpha; CaM-KI; CAMK1-PEN; CaMKI; CaMKI-alpha; G alpha interacting protein; regulator of G-protein signalling 19
Gene Aliases: AI505105; CAMK1; CAMKI; CaMKIalpha; D6Ertd263e; Gaip
Molecular Function: non-receptor serine/threonine protein kinase
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