|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Western Blot (WB)||1:25|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human calbindin D28K protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is predicted to react with bovine, chicken, mouse and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is kidney or cerebellum tissue.
Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28kDa calcium binding protein, with broad tissue distribution. It belongs, together with calmodulin, S-100, parvalbumin, trponin C and other proteins, to a family of low molecular weight calcium binding proteins (CaBPs). These CaBPs have homologous primary structures, which contain conserved polypeptide folds of the EF-hand type for Ca2+ binding. Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CALB; Calbindin; calbindin 1, (28kD); calbindin 1, 28kDa; Calbindin D28; D-28K; RTVL-H protein; Vitamin D-dependent calcium-binding protein, avian-type
Gene Aliases: CAB27; CALB; CALB1; D-28K
UniProt ID: (Human) P05937
Entrez Gene ID: (Human) 793
Molecular Function: calcium-binding protein
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