|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||2µg per 10^6 cells|
|Western Blot (WB)||1:500|
|Blocking Assay (BLOCK)||See 1 publications below|
|Western Blot (WB)||See 3 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Bacteria, Bovine, Chicken, Human, Mouse, Rat|
|Published species reactivity||Fungi , Rat , Shrew , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Calmodulin purified from Dictyostelium discoideum.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-917 detects calmodulin from human, bovine, chicken, Chlamydomonas, Dictyostelium, mouse and rat samples. This antibody does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).
MA3-917 has been successfully used in Western blot, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects a 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Addition of EGTA to buffers completely inhibits antibody staining. Immunohistochemical staining of calmodulin in Dictyostelium cells with MA3-917 results in staining of the contractile vacuoles.
The MA3-917 antigen is calmodulin purified from Dictostelium discoideum.
Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CALM 1; calmodulin; Calmodulin 1 (phosphorylase kinase, delta); calmodulin 2 (phosphorylase kinase, delta); calmodulin-like protein (neoCaM); caM; Neo-calmodulin; NeoCaM; PHKD; Phosphorylase Kinase Delta; phosphorylase kinase subunit delta; phosphorylase kinase, delta subunit; prepro-calmodulin 1
Gene Aliases: AI256814; AI327027; AI461935; AL024000; BOS_19653; Calm; CALM2; CALML2; CaM; CAMI; CPVT4; DD132; LQT14; PHKD
UniProt ID: (Chicken) P05419
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