|ELISA (ELISA)||0.5-1.0 ug/ml|
|Immunoprecipitation (IP)||1-2 µg/ml|
|Western Blot (WB)||0.5-1 µg/ml|
|Tested Species reactivity||Bovine, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human Calpain protein purified from placenta.|
|Storage buffer||PBS, pH 7.5, with 50% glycerol|
|Contains||3mM sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Calcium-activated neutral proteinase 1; Calcium-activated neutral proteinase 2; calpain 1, (mu/I) large subunit; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; Calpain large polypeptide L2; Calpain M-type; Calpain mu-type; calpain, large polypeptide L1; calpain, large polypeptide L2; Calpain-1 catalytic subunit; Calpain-1 large subunit; Calpain-2 catalytic subunit; Calpain-2 catalytic subunit (EC:188.8.131.52); Calpain-2 large subunit; CANP 1; CANP 2; CANP1; CANP2; CANPL1; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; M-calpain; micromolar calcium activated neutral protease 1; micromolar calcium-activated neutral protease 1; Micromolar-calpain; Millimolar-calpain; mu-calpain; muCANP; muCL
Gene Aliases: BOS_15643; BOS_24946; CANP; CANP1; CANP2; CANPL1; CANPL2; CANPml; CAPN1; CAPN2; mCANP; muCANP; muCL; PIG30; SPG76
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