|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from internal region of human calpain protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast ductal carcinoma.
Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both Calpain I and II and is associated with myotonic dystrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 30K; Calcium-activated neutral proteinase small subunit; calcium-dependent protease; Calcium-dependent protease small subunit; Calcium-dependent protease small subunit 1; calcium-dependent protease, small subunit; calpain; calpain 4; calpain 4, small subunit (30K); Calpain regulatory subunit; Calpain small subunit 1; calpain, small polypeptide; calpain, small subunit 1; CALPAIN4; CANP small subunit; CANPS; CAPN4; CDPS; CSS1; small pol; small subunit; small subunit 30K
Gene Aliases: CALPAIN4; CANP; CANPS; CAPN4; CAPNS; CAPNS1; CDPS; CSS1
UniProt ID: (Human) P04632
Entrez Gene ID: (Human) 826
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