|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||1:50 - 1:200|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000|
|Western Blot (WB)||See 76 publications below|
|Immunocytochemistry (ICC)||See 50 publications below|
|Flow Cytometry (Flow)||See 3 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunoprecipitation (IP)||See 14 publications below|
|Immunohistochemistry (IHC)||See 9 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Tested Species reactivity||Dog, Human, Mouse, Non-human primate, Rabbit, Rat|
|Published species reactivity||Dog , Rat , Non-human primate , Virus , Amphibian , Bovine , Hamster , Mouse , Human , Echinoderm|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant, human calreticulin protein.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA3-900 detects calreticulin from human, non-human primate, rabbit, mouse, canine and rat samples.
PA3-900 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~60 kDa protein representing calreticulin from rat brain extract. Immunofluorescence staining of calreticulin in rat brain cortex with PA3-900 results in fine granular staining consistent with ER localization.
PA3-900 immunogen is recombinant human calreticulin protein produced in the Baculovirus insect cell system.
Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. This protein was originally identified in SR membranes and plays a minor role in calcium storage in skeletal and cardiac muscle SR. Calreticulin is also known as calregulin, CRP55, CaBP3, calsequestrin-like protein and Ro/SS-A antigen. Calreticulin binds calcium with low affinity and high capacity, however it also exhibits a single high affinity binding site. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of calreticulin and other resident ER proteins including glucose regulated protein 78 (GRP78), GRP94 and protein disulfide isomerase (PDI). This sequence is responsible for the retention of newly synthesized proteins within the ER lumen. This retention is reported to be mediated by a KDEL receptor. Recent reports indicate that calreticulin can act as a modulator of the regulation of gene transcription by nuclear hormone receptors and may also act as a molecular chaperone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CABP3; CALBP; Calcium-binding protein 3; Calr; Calregulin; Calreticulin; Calsequestrin-Like Protein; CRP55; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; grp60; HACBP; Ro/SS-A Antigen; Sicca syndrome antigen A (autoantigen Ro; calreticulin)
Gene Aliases: CALR; Calregulin; cC1qR; CRT; CRTC; HEL-S-99n; RO; SSA
Molecular Function: calcium-binding protein
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