|Flow Cytometry (Flow)||0.5-1 ug/test|
|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||0.1-0.2 ug/ml|
|Immunocytochemistry (ICC)||See 3 publications below|
|Western Blot (WB)||See 3 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Pig , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 171-175 of P42574.|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with bovine, canine, feline, hamster, mouse, primate, pufferfish, rabbit, rat, porcine and Xenopus based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Caspases are a family of cysteine proteases that centrally controls apoptotic machinery. Caspases can be grouped according to their substrate specificities that are largely determined by the amino acids preceding the cleavage site. One group of caspases that include -6, -8, is specific for the substrate V/LEXD. This substrate is a site similar to those found in caspase proenzymes. This group of caspases may function as initiators of a proteolytic cascade by activating pro-caspases to amplify a death signal. A second group of caspases is specific for the substrate DEXD that is related to sites found on target proteins cleaved during apoptosis.
Caspase-3 is a member of the interleukin-1 beta converting enzyme, Caspase-8, nuclear lamins and others. The overexpression of Caspase-3 can result in apoptosis. Likewise, the inhibition of Caspase-3 or other caspases can prevent cells from entering the apoptotic pathway. Recent evidence has revealed a link between plasma caspase-3 and atherosclerosis and it role in activation of apoptosis in breast cancer mediated by siRNA-mediated Apollon silencing. This antibody is specific for the cleaved (active) form of caspase-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Apopain; apoptosis-related cysteine protease; CASP-3; caspase 3; caspase 3, apoptosis related cysteine protease; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; CPP-32; Cysteine protease CPP32; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; Yama
Gene Aliases: A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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