|Immunohistochemistry (Paraffin) (IHC (P))||1:1000|
|Western Blot (WB)||1:500|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The epitope corresponding to amino acids 217-350 mapping within the caspase-8 p18 subunit of human origin|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
PA1-29159 detects Caspase 8 from human, mouse, rat samples.
PA1-29159 has been successfully used in IF/ICC, immunohistochemistry (paraffin tissue), immunoprecipitation, and Western blot applications.
The PA1-29159 immunogen corresponds to amino acids 217-350 mapping within the caspase-8 p18 subunit of human origin.
Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the proform of the Caspase 8 protein. Recruitment of Caspase 8 to the Fas receptor results in oligomerization of the Caspase 8 protein, which in turn drives up its autoactivation through "self-cleavage". Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby commiting the cell to undergo apoptosis. Reacts with Jurkat cells and Tonsil.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; CASP-8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; Caspase-8; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; Fas-linked ICE-like protease; FLICE; ICE-like apoptotic protease 5; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; Mch5; MORT1-associated ced-3 homolog
Gene Aliases: ALPS2B; CAP4; CASP-8; CASP8; FLICE; MACH; MCH5
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