|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:250|
|Western Blot (WB)||1:500-1:1000|
|Immunohistochemistry (IHC)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Sheep Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human Caspase 9 pro-domain protein.|
|Contains||0.1% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
Suggested positive control: HEK 293 whole cell lysate.
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase-9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: APAF-3; APAF3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; cas9; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase-9; CASPASE-9c; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; protein phosphatase 1, regulatory subunit 56; RP11-265F14.3
Gene Aliases: APAF-3; APAF3; CASP9; ICE-LAP6; MCH6; PPP1R56
UniProt ID: (Human) P55211
Entrez Gene ID: (Human) 842
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