|Hemagglutination Assay (HA)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||10µg/mg protein lysate|
|Western Blot (WB)||2-4 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunohistochemistry (IHC)||See 5 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Bovine, Human, Mouse, Sheep, Rat|
|Published species reactivity||Rat , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding aa 1-134 of human caspase 9|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
PA5-16358 targets Caspase 9 in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Bovine, Human, mouse, Ovine, and Rat samples.
The PA5-16358 immunogen is recombinant protein encoding aa 1-134 of human caspase 9.
Activation of procaspase-9 by Apaf-1 in the cytochrome c/dATP-dependent pathway requires proteolytic cleavage to generate the mature caspase molecule. Deletion of the Apaf-1 WD-40 repeats makes Apaf-1 constitutively active and capable of processing procaspase-9 independent of cytochrome c an dATP. Apaf-1-mediated processing of procaspase-9 occurs at Asp-315 by an intrinsic autocatalytic activity of procaspase-9 itself. Apaf-1 can form oligomers and may facilitate procaspase-9 autoactivation by oligomerizing its precursor molecules. Once activated, caspase-9 can initiate a caspase cascade involving the downstream executioners caspase-3, -6, and -7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 25 kDa caspase-9 dominant negative protein; APAF-3; apoptosis-related cysteine protease; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; cas9; CASP-9; caspase 9; caspase 9, apoptosis-related cysteine peptidase; Caspase-9; caspase-9 dominant negative form; caspase-9-carboxyl-terminal divergent; ICE-LAP6; ICE-like apoptotic protease 6; protein phosphatase 1, regulatory subunit 56
Gene Aliases: AI115399; APAF-3; APAF3; AW493809; BOS_16018; CASP-9; Casp-9-CTD; CASP9; Casp9_v1; Caspase-9; ICE-LAP6; MCH6; PPP1R56
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