|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||1-2 µg/ml|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Western Blot (WB)||See 5 publications below|
|Tested Species reactivity||Dog, Hamster, Human, Mouse, Rat|
|Published species reactivity||Rat , Human Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) S G G K Y V D S E G H L Y T V P(17) C of human CAV1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-064 detects caveolin 1 from human, canine, hamster, mouse, and rat tissues and cells.
PA1-064 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~22 kDa protein representing caveolin 1 from rat heart protein extract.
The PA1-064 immunogen is a synthetic peptide corresponding to residues M(1) S G G K Y V D S E G H L Y T V P(17) C of human CAV1. This sequence is completely conserved between human, mouse, canine, bovine, and rat. The PA1-064 immunizing peptide (Cat. # PEP-146) is available for use in neutralization and control experiments.
Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CAV; CAV1; caveolin 1, caveolae protein, 22kDa; caveolin caveolae protein 22 kDa; caveolin, caveolae protein 1; caveolin, caveolae protein, 22 kDa; Caveolin-1; cell growth-inhibiting protein 32; Vesicular integral-membrane protein VIP21
Gene Aliases: BSCL3; CAV; Cav-1; CAV1; caveolin 1; CGL3; LCCNS; MSTP085; PPH3; VIP21
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