|ELISA (ELISA)||Assay dependent|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||5 µg/ml|
|Immunoprecipitation (IP)||2.5 µg|
|Radioimmune Assays (RIA)||Assay dependent|
|Western Blot (WB)||1 µg/ml|
|Immunoprecipitation (IP)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Rat, Human, Mouse, Amphibian, Xenopus|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Carboxyterminal fragment starting at aa 85 of Xenopus p34cdc2 expressed in E. coli|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-21453 detects Cdc2 (CDK1) in human, rat, mouse, xenopus laevis, and amphibian samples.
MA1-21453 has been successfully used in ELISA, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, radioimmune assays, and Western Blot procedures.
MA1-21453 immunogen corresponds to Carboxyterminal fragment starting at aa 85 of Xenopus p34cdc2 expressed in E. coli.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Cdc2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In the fission yeast Schizosaccharomyces pombe, the gene Cdc2 is responsible for controlling the transition from G1 phase to the S phase and from the G2 phase to the M phase of the cell cycle. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34-cyclin B complex that is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; cell division control protein 2 homolog; Cell division control protein 2 homolog 1; cell division control protein 2-A; cell division cycle 2 homolog A; cell division cycle 2 like; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; cell division protein kinase 1; cell division protein kinase 1-A; cyclin-dependent kinase 1; cyclin-dependent kinase 1-A; p34 protein kinase; p34 protein kinase 1
Gene Aliases: cdc-2; CDC2; cdc2-a; CDC28A; Cdc2a; cdc2x1.1; p34<CDC2>; P34CDC2; PSTAIR; xcdc2
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