|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:50 - 1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant, full length human Cdc25B protein.|
|Storage conditions||4° C|
MA1-26441 detects Cdc25B from human samples.
MA1-26441 has been successfully used in immunohistochemistry (frozen and paraffin), and immunoprecipitation procedures. Formalin-fixed paraffin embedded tissues require boiling in 10mM citrate buffer (pH 6.0) prior to immunostaining.
The MA1-26441 immunogen is recombinant, full length human protein.
The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and Cdc25 phosphatases. Three Cdc25 genes are present in human cells: Cdc25A, Cdc25B and Cdc25C. Cdc25A and Cdc25B are expressed throughout the cell cycle, with peak expression in G1 for Cdc25A and in both G1-S phase and G2 for Cdc25B. Cdc25C is mainly expressed in G2 phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: dual specificity phosphatase Cdc25B; M-phase inducer phosphatase 2
Gene Aliases: RP5-1009E24.3
UniProt ID: (Human) P30305
Entrez Gene ID: (Human) 994
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