|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay dependent|
|Immunohistochemistry (IHC)||See 15 publications below|
|Western Blot (WB)||See 6 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Dog , Rabbit , Rat , Mouse Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||BALB/C mice were injected with recombinant human CDC47 protein.|
|Storage conditions||4° C|
MA1-91554 detects CDC47 from human samples.
MA1-91554 has been successfully used in immunohistochemistry (paraffin tissue), immunoprecipitation, Western blot applications.
The MA1-91554 immunogen is: BALB/C mice were injected with recombinant human CDC47 protein.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CDC47 homolog; Cyclin dependent kinase 47; DNA replication licensing factor MCM7; homolog of S. cerevisiae Cdc47; minichromosome maintenance deficient 7
Gene Aliases: CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146
UniProt ID: (Human) P33993
Entrez Gene ID: (Human) 4176
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