|ChIP assay (ChIP)||1-3 ul|
|Immunofluorescence (IF)||Assay dependent|
|Immunoprecipitation (IP)||10 µg/mg of lysate|
|Western Blot (WB)||1-2 µg/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide: C-SQQPPQYSHQTHRY, corresponding to amino acids 451-464 of Human Cdk8.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, do not freeze|
PA1-21780 detects Cdk8 in Human, Rat and Mouse samples.
PA1-21780 has been successfully used in ChIP, Immunofluorescence, Immunoprecipitation and Western Blot procedures.
PA1-21780 immunogen corresponds to Synthetic peptide: C-SQQPPQYSHQTHRY, corresponding to amino acids 451-464 of Human Cdk8..
A family of proteins designated as cdks are critical regulators of cell cycle progression. The prototype member of this family, p34 / Cdc 2, and a related protein Cdk 2, function late in the cycle while Cdk 4 and Cdk 6 are critically involved in G1 to S progression. The p54 / Cdk 8 interacts exclusively with Cyclin C. The Cdk 8 Cyclin C complex activates the kinase. Because of analogy with yeast Cdk 8, it has been proposed that the human Cdk 8 Cyclin C may interact with RNA polymerase, thus influencing the transcription mechanism(s) that lead to cell division.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CDK8 protein kinase; Cell division protein kinase 8; Cyclin-dependent kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; MGC126074; MGC126075; Protein kinase K35
Gene Aliases: CDK8; K35
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