|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:1000|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE)|
|Storage buffer||whole serum diluted in 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-32113 detects CDK9 from mouse, rat, human samples.
PA1-32113 has been successfully used in immunoprecipitation, immunohistochemistry (paraffin tissue), ELISA and Western blot applications.
The PA1-32113 immunogen is multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE).
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C-2K; CDC2-related kinase; CDC2L4; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; CTK1; Cyclin-dependent kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); PITALRE; RP11-228B15.5; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; TAK; Tat-associated kinase complex catalytic subunit
Gene Aliases: C-2k; CDC2L4; CDK9; CTK1; PITALRE; TAK
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