|Flow Cytometry (Flow)||1/200|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunomicroscopy (IM)||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100-1:500|
|Western Blot (WB)||See 12 publications below|
|Immunocytochemistry (ICC)||See 48 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 8 publications below|
|Blocking Assay (BLOCK)||See 2 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Bovine, Hamster, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Dog , Rat , Non-human primate , Hamster , Bovine , Human , Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified human brain clathrin heavy chain.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-065 detects clathrin heavy chain in non-human primate, bovine, human, rat and mouse tissues as well as hamster (CHO) cells.
MA1-065 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody recognizes a single ~180 kDa protein representing the clathrin heavy chain from bovine brain extract. Immunoprecipitation of triskelions with MA1-065 allows both the clathrin heavy chain and the associated light chain polypeptides to be examined.
The MA1-065 immunogen is purified human brain clathrin heavy chain. Electron microscopy and proteolysis mapping demonstrate that MA1-065 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CHC17; Clathrin heavy chain 1; Clathrin heavy chain on chromosome 17; Clathrin heavy polypeptide; clathrin, heavy chain (Hc); clathrin, heavy polypeptide (Hc); clathrin, heavy polypeptide-like 2; CLH-17; CLH17; CLTC; KIAA0034
Gene Aliases: 3110065L21Rik; BOS_18343; CHC; CHC17; CLH-17; CLH17; CLTC; CLTCL2; Hc; KIAA0034; R74732
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