|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||2-5 ug/ml|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Bovine, Human, Mouse, Pig, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgM|
|Storage buffer||TBS, pH 8.0|
|Contains||15mM sodium azide|
|Storage conditions||4° C, do not freeze|
Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CHC; CHC17; clathrin; clathrin heavy chain; Clathrin heavy chain 1; Clathrin heavy chain on chromosome 17; clathrin, heavy chain (Hc); clathrin, heavy polypeptide (Hc); clathrin, heavy polypeptide-like 2; CLH-17; CLTCL2; Hc; heavy polypeptide (Hc); heavy polypeptide-like 2; KIAA0034
Gene Aliases: 3110065L21Rik; BOS_18343; CHC; CHC17; CLH-17; CLH17; CLTC; CLTCL2; Hc; KIAA0034; R74732
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