|Immunohistochemistry (Paraffin) (IHC (P))||2-4 ug/ml|
|Western Blot (WB)||1:500-1:5000|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Western Blot (WB)||See 1 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 224-237 of P56856.|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with chimpanzee, equine, rat and Rhesus monkey based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Claudin-18 is a tight junction protein expressed as lung-and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene. The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor. A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human. Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein. Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells. These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach. Claudin-18 expression has also been reported in the inner ear. Because of its highly restricted expression pattern in normal tissues and frequent activation in numerous human cancers, claudin-18 may represent an important target for antibody therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Claudin-18; CLDN18; pulmonary associated protein J; SFTA5; SFTPJ; surfactant; surfactant associated 5; surfactant associated protein J; surfactant, pulmonary associated protein J
Gene Aliases: CLDN18; SFTA5; SFTPJ; UNQ778/PRO1572
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